THE PRODUCTION OF D-ACETOIN BY A TRANSGENIC ESCHERICHIA-COLI

Authors
UI S MIMURA A OKUMA M KUDO T
Citation
S. Ui et al., THE PRODUCTION OF D-ACETOIN BY A TRANSGENIC ESCHERICHIA-COLI, Letters in applied microbiology, 26(4), 1998, pp. 275-278
Citations number
16
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Letters in applied microbiology → ACNP
ISSN journal
02668254
Volume
26
Issue
4
Year of publication
1998
Pages
275 - 278
Database
ISI
SICI code
0266-8254(1998)26:4<275:TPODBA>2.0.ZU;2-A
Abstract
A 6 kbp SphI fragment encoding genes for the enzymes alpha-acetolactat e synthase (ALS), alpha-acetolactate decarboxylase (ALDC) and meso-2,3 -butanediol dehydrogenase (meso-BDH), involved in the formation of mes o-2,3-butanediol (meso-BD) from pyruvic acid, was cloned into plasmid pUC118. When derivatives of this plasmid were introduced into Escheric hia coli JM109, the transformants were able to produce D-acetoin (D-AC ) without contamination with L-acetoin (L-AC).