HYDROQUINONE-O,O'-DIACETIC ACID AS A MORE LABILE REPLACEMENT FOR SUCCINIC ACID LINKERS IN SOLID-PHASE OLIGONUCLEOTIDE SYNTHESIS

Authors
PON RT YU SY
Citation
Rt. Pon et Sy. Yu, HYDROQUINONE-O,O'-DIACETIC ACID AS A MORE LABILE REPLACEMENT FOR SUCCINIC ACID LINKERS IN SOLID-PHASE OLIGONUCLEOTIDE SYNTHESIS, Tetrahedron letters, 38(19), 1997, pp. 3327-3330
Citations number
15
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Tetrahedron letters → ACNP
ISSN journal
00404039
Volume
38
Issue
19
Year of publication
1997
Pages
3327 - 3330
Database
ISI
SICI code
0040-4039(1997)38:19<3327:HAAAML>2.0.ZU;2-P
Abstract
Hydroquinone-O,O'-diacetic acid (QDA) can be used to link nucleosides to CPG or polystyrene supports instead of succinic acid. Cleavage of o ligodeoxy- or oligoribonucleotides, using ammonium hydroxide, requires only two to five minutes. The QDA linker is stable, easily prepared a nd does not require any other changes to the reagents, methods, or ins trumentation used in automated solid-phase oligonucleotide synthesis. (C) 1997 Elsevier Science Ltd.