RAPID AUTOMATED DERIVATIZATION OF SOLID-PHASE SUPPORTS FOR OLIGONUCLEOTIDE SYNTHESIS USING URONIUM OR PHOSPHONIUM COUPLING REAGENTS

Authors
PON RT YU SY
Citation
Rt. Pon et Sy. Yu, RAPID AUTOMATED DERIVATIZATION OF SOLID-PHASE SUPPORTS FOR OLIGONUCLEOTIDE SYNTHESIS USING URONIUM OR PHOSPHONIUM COUPLING REAGENTS, Tetrahedron letters, 38(19), 1997, pp. 3331-3334
Citations number
25
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Tetrahedron letters → ACNP
ISSN journal
00404039
Volume
38
Issue
19
Year of publication
1997
Pages
3331 - 3334
Database
ISI
SICI code
0040-4039(1997)38:19<3331:RADOSS>2.0.ZU;2-K
Abstract
Very fast (4 sec) attachment of nucleosides to CPG supports, via succi nyl or QDA linkers, is possible with either uronium (HBTU, HATU, HBPyU , HBPipU) or phosphonium (BOP, PyBOP(R), PyBroP, BroP) coupling reagen ts and automated derivatization of CPG and polystyrene supports just p rior to oligonucleotide synthesis has been demonstrated. (C) 1997 Else vier Science Ltd.