SYNTHESIS OF A VERSATILE PURIFICATION HANDLE FOR USE WITH BOC CHEMISTRY SOLID-PHASE PEPTIDE-SYNTHESIS

The synthesis of a versatile handle for the purification of synthetic peptides is described. The Boc-aminoethylsulfonylethyloxycarbonyl hand le is coupled to the NH2-terminus of the resin-bound peptide. Removal of the Boc group affords a free amine which can be derivatized with an y of a variety of functionalities. Cleavage from the resin gives a pep tide functionalized for covalent chemoselective reaction with the colu mn support. Desired full length peptide is eluted from the column by c leavage of the handle by treatment with base. (C) 1997 Elsevier Scienc e Ltd.