QUALITATIVE AND QUANTITATIVE MEASUREMENTS OF OLIGONUCLEOTIDES IN GENE-THERAPY .1. IN-VITRO MODELS

Part I of this review attempts to bring together all the methods of de tection and determination of synthetic oligonucleotides used in in vit ro, described in the literature over the past 14 years, in an effort b y scientists to use these oligonucleotides as drugs in gene therapy. T he in vitro models include cell-free and cell culture systems. Emphasi s has been given to the techniques developed for quantification of the input oligonucleotides or their metabolites. The purpose of study, me thods of processing, detection and determination techniques such as th ose based on fluorescence, radiolabeling, high-performance liquid chro matography, gel-elecrrophoresis and others have been presented. (C) 19 97 Elsevier Science B.V.