DIRECT AND CONTINUOUS ELECTROCHEMICAL MEASUREMENT OF NORADRENALINE-INDUCED NITRIC-OXIDE PRODUCTION IN C6 GLIOMA-CELLS

1. Nitric oxide (NO) production in C6 glioma cells was directly monito red in real time by electrochemical detection with a NO-specific biose nsor. 2. We present here the first direct evidence that noradrenaline elicits long-lasting NO production in C6 cells pretreated with lipopol ysaccharide and interferon-gamma, an effect blocked by N-G-monomethyl- L-arginine, a NO synthase inhibitor. 3. This direct electrochemical me asurement of glia-derived NO should facilitate our understanding of th e kinetics of glial signaling in glia-glia and glia-neuron networks in the brain.