F. Uysal et al., EFFECT OF VITAMIN-E ON ANTIOXIDANT ENZYMES AND NITRIC-OXIDE IN ISCHEMIA-REPERFUSED KIDNEY INJURY, Biochemistry and molecular biology international, 44(6), 1998, pp. 1255-1263
The effects of Vitamin E administration on antioxidant enzyme activiti
es and nitrite-nitrate levels of the reperfused rat kidney tissues wer
e investigated by performing a 60 min ischemia followed by 24 and 72 h
ours of reperfusion. Vitamin E administration or the placebo (SF) was
applied as 100 mg/kg BW. As expected, catalase (CAT) (p<0.05) and supe
roxide dismutase (SOD) (p<0.05) activities of ischemia/reperfused (I/R
) kidney tissue were lower and malondialdehyde (MDA) levels were highe
r than control kidneys in both SF and vitamin E treated groups followi
ng 24 h reperfusion. During reperfusion of long term (72 h), vitamin E
triggered a decrease in the MDA levels in the ischemic tissue, while
it did not provoke a significant effect on SOD and catalase activities
. Total nitrite levels of ischemic tissues in both of the groups were
higher than matched control kidneys and this elevation was more clear
in the vitamin E treated group. Our-results showed that vitamin E has
a protective effect on I/R injury, by a direct chain breaking effect o
n lipid peroxidation (LPO) and hence preventing the nitric oxide (NO)
reservoir of ischemic tissue. Alfa-tocopherol may be a promising agent
for the prevention of tissue injury caused by free oxygen radicals.