TRANSMISSION OF HEPATITIS-C VIRUS-INFECTION TO TREE SHREWS

Although hepatitis C virus (HCV) infection can be reproduced in chimpa nzees, these animals are rare and expensive. Tree shrews (tupaias) are small animals, closely related to primates, which adapt easily to a l aboratory environment In this work we have investigated the susceptibi lity of Tupaia belangeri chinensis to HCV infection. Tupaias caught in the wild in Yunnan (China) were inoculated in China with HCV genotype 1b (study A) and in Spain with a mixture of genotypes 1b, 1a, and 3 ( study B). In study B tupaias were divided into three groups: group I w as inoculated without previous manipulation, group II received 750 cGy of X-ray whole-body irradiation before inoculation, and group III was used as control. Transient or intermittent viremia occurred in 34.8% (8/23) and anti-HCV in 30.4% (7/23) of tupaias in study A In study 8 a transient viremia was detected in 20% (2/10) in group I and in 50% (2 /4) in group II. Anti-HCV was found in 1 tupaia from group I and in 3 from group II. Viremia lasted for longer and anti-HCV tended to reach higher titers in animals which received total body irradiation. ALT el evations and nonspecific pathological changes occurred in inoculated t upaias; however, the wild nature of the animals precludes the interpre tation of these changes as solely due to HCV infection. In summary our results show that T. b. chinensis are susceptible to HCV and that who le-body irradiation may possibly increase the efficiency of the infect ion. These animals may serve as an in vivo system for culturing HCV an d addressing pathophysiological and therapeutic issues of HCV infectio n. (C) 1998 Academic Press.