PROTECTIVE EFFECT OF CEFTRIAXONE AGAINST THE NEPHROTOXICITY OF ISEPAMICIN ADMINISTERED ONCE-DAILY IN RATS

The protective effect of ceftriaxone on isepamicin-induced nephrotoxic ity was investigated. For 14d, Wistar rats were administered either ce ftriaxone 100 mg/kg intraperitoneally isepamicin 300 mg/kg subcutaneou sly, or ceftriaxone isepamicin in combination. The animals given 300 m g/kg of isepamicin showed a significant increase in urine NAC (N-acety l-beta-D-glucosaminidase) levels as compared with the control animals which received saline (p < 0.01). However, the increase in NAC level w as markedly less when isepamicin Was administered in combination with ceftriaxone (p<0.01). Ceftriaxone alone had no effect on urine NAG act ivity. Serum creatinine levels were significantly higher in animals tr eated with isepamicin alone than in control animals (p<0.01) or animal s receiving the isepamicin ceftriaxone combination (p<0.01). lifter 14 d of treatment, ceftriaxone had not accumulated in renal tissue, but it did reduce the renal intracortical accumulation of isepamicin (p<0. 01), Histopathologically ceftriaxone induced very few cellular alterat ions and considerably reduced the manifestation of typical signs of is epamicin nephrotoxicity. This investigation demonstrates that ceftriax one protects animals against isepamicin-induced nephrotoxicity.