DIRECT QUANTITATIVE-ANALYSIS OF LINAMARIN IN CASSAVA BY HIGH-PERFORMANCE THIN-LAYER CHROMATOGRAPHY

A simple, rapid, and accurate HPTLC quantitative procedure is presente d for the direct determination of linamarin. A cassava sample was extr acted with boiling 80% v/v methanol and the extract and linamarin stan dard were applied to silica gel HPTLC plates prewashed by development with methanol, The plates were first developed to a distance of 30 mm with ethyl acetate-acetone-water, 40 + 50 + 10 (v/v), then to a distan ce of 85 mm with ethyl acetate-formic acid-water, 60 + 10 + 10 (v/v). The spots were visualized by dipping the plate into a solution of anil ine (2%), diphenylamine (2%), and phosphoric acid 15% in acetone, then heating at 105 degrees C for 60 min, Densitometric quantification was effected at lambda = 525 nm by transmission scanning. The method,sas validated for accuracy, intraday, and interday reproducibility of peak area, linearity, and detection limit.