A. Tybjaerghansen et al., ASSOCIATION OF MUTATIONS IN THE APOLIPOPROTEIN-B GENE WITH HYPERCHOLESTEROLEMIA AND THE RISK OF ISCHEMIC-HEART-DISEASE, The New England journal of medicine, 338(22), 1998, pp. 1577-1584
Background Familial hypercholesterolemia leads to premature ischemic h
eart disease and is often caused by mutations in the gene for the low-
density lipoprotein receptor. Mutations in the apolipoprotein B gene,
which encodes a ligand for this receptor, may also result in this phen
otype, Methods We studied the genotypes of 9255 women and men from the
general population, 948 patients with ischemic heart disease, and 36
patients with familial hypercholesterolemia, all from Denmark, for thr
ee mutations in the apolipoprotein B gene: Arg3500Gln, Arg3531Cys, and
Arg3500Trp. Results The prevalence of heterozygotes in the general po
pulation was 0.08 percent (95 percent confidence interval, 0.03 to 0.1
6 percent) for both the Arg3500Gln and the Arg3531Cys mutations, and 0
.00 percent (95 percent confidence interval, 0.00 to 0.18 percent) for
the Arg3500Trp mutation. Among carriers of the Arg3500Gln mutation, c
holesterol levels were significantly higher than among noncarriers in
the general population - by 100 mg per deciliter (2.6 mmol per liter)
among carriers in the general population, 154 mg per deciliter (4.0 mm
ol per liter) among patients with ischemic heart disease, and 172 mg p
er deciliter (4.5 mmol per liter) among patients with familial hyperch
olesterolemia. Heterozygous carriers of the Arg3500Gln mutation were s
ignificantly more common among patients with ischemic heart disease (o
dds ratio, 7.0; 95 percent confidence interval, 2.2 to 22; P=0.003) an
d patients with familial hypercholesterolemia (odds ratio, 78; 95 perc
ent confidence interval, 16 to 388; P=0.001) than in the general popul
ation. Heterozygous carriers of the Arg3531Cys mutation in the general
population did not have higher-than-normal plasma cholesterol levels
or an increased risk of ischemic heart disease (odds ratio; 1.4; 95 pe
rcent confidence interval, 0.2 to 11; P=0.54), Conclusions The Arg3500
Gln mutation in the apolipoprotein B gene, which is responsible for fa
milial defective apolipoprotein B-100 and is present in approximately
1 in 1000 persons in Denmark, causes severe hypercholesterolemia and i
ncreases the risk of ischemic heart disease. (C) 1998, Massachusetts M
edical Society.