A HIGH-COPY-NUMBER ADE2-BEARING PLASMID FOR TRANSFORMATION OF CANDIDA-GLABRATA

The Candida glabrata ADE2 gene encoding aminoimidazole ribonucleotide (AIR) carboxylase (EC 4.1.1.21) was isolated by complementation of the ade2-1 mutation in Saccharomyces cerevisiae. The predicted amino acid (aa) sequence is 75% identical to that of S. cerevisiae. Integrative transformation was used to produce a C. glabrata strain bearing a dele tion of ADE2 coding sequences. A high-copy-number shuttle vector beari ng the ADE2 gene was constructed and contains a fragment of S. cerevis iae mitochondrial (mt) DNA that confers the ability to replicate auton omously in C. glabrata. (C) 1998 Published by Elsevier Science B.V. Al l rights reserved.