SITE-SPECIFIC ENDONUCLEASE SSCL1-I FROM STRAIN STAPHYLOCOCCUS SPECIESL1

A site-specific endonuclease SscL1 I preparation has been isolated and purified to near homogeneity from the strain Staphylococcus sp. L1 wi thout admixtures of other nuclease activity. DNA cleavage proceeds acc ording to the scheme: 5'-G down arrow ANT C-3' 3'-C TNA up arrow G-5', and thus the isolated enzyme is an isoschizomer of restriction endonu clease HinfI and belongs to the second class of restriction endonuclea ses. SscL1 I works over a broad range of temperature and pH. The enzym e is characterized by high stability during storage.