STRUCTURE REFINEMENT AGAINST SYNCHROTRON LAUE DATA - STRATEGIES FOR DATA-COLLECTION AND REDUCTION

The synchrotron Laue technique has been applied to high-resolution str ucture refinement of the ribotoxin, restrictocin [Yang & Moffat (1996) . Structure, 4, 837-852]. By employing carefully designed data-collect ion strategies and the data-reduction algorithms incorporated in the s oftware system LaueView [Ren & Moffat (1995a). J. Appl. Cryst. 28, 461 -481; Ren & Moffat (1995b). J. Appl. Cryst. 28, 482-493], a set of hig h-resolution Laue data with a completeness and accuracy comparable to excellent monochromatic data was obtained. Through detailed comparison with the monochromatic data and electron-density maps derived from th e Laue data, optimum data-collection and reduction strategies were ide ntified and the application of Laue diffraction techniques to conventi onal crystallographic refinement was demonstrated.