CRYSTALLIZATION AND PRELIMINARY-X-RAY STUDIES ON THE HYPERSTABLE 3-ISOPROPYLMALATE DEHYDROGENASE FROM THE THERMOACIDOPHILIC ARCHAEON SULFOLOBUS SP. STRAIN-7

Authors
SUZUKI T MORIYAMA H HIROSE R SAKURAI M TANAKA N OSHIMA T
Citation
T. Suzuki et al., CRYSTALLIZATION AND PRELIMINARY-X-RAY STUDIES ON THE HYPERSTABLE 3-ISOPROPYLMALATE DEHYDROGENASE FROM THE THERMOACIDOPHILIC ARCHAEON SULFOLOBUS SP. STRAIN-7, Acta crystallographica. Section D, Biological crystallography, 54, 1998, pp. 444-445
Citations number
13
Categorie Soggetti
Crystallography,"Biochemical Research Methods",Biophysics,Biology
Journal title
Acta crystallographica. Section D, Biological crystallography → ACNP
ISSN journal
09074449
Volume
54
Year of publication
1998
Part
3
Pages
444 - 445
Database
ISI
SICI code
0907-4449(1998)54:<444:CAPSOT>2.0.ZU;2-Q
Abstract
3-Isopropylmalate dehydrogenase from the thermoacidophilic archaeon, S ulfolobus sp. strain 7, has been crystallized by the vapor-diffusion m ethod. The crystals were grown from a solution containing ammonium sul fate, 2-methyl-2,4-pentanediol and magnesium chloride. The crystalliza tion requires 2-methyl-2,4-pentanediol to avoid twinning of the crysta ls. The crystal belongs to the orthorhombic system with the space grou p P222(1) and unit-cell dimensions a = 67.9, b = 93.3 and c = 134.1 An gstrom.