DISCRIMINATION OF EUROPEAN AND AUSTRALIAN GLOBODERA-ROSTOCHIENSIS ANDGLOBODERA-PALLIDA PATHOTYPES BY HIGH-PERFORMANCE CAPILLARY ELECTROPHORESIS

High performance capillary electrophoresis (CE) is a family of related microanalytical preparative techniques for the rapid separation of pr oteins, peptides oligonucleotides, and oligosaccharides. This article describes the use of CE as a tool to differentiate between representat ive populations of pathotypes of the potato cyst nematode species Glob odera rostochiensis and G. pallida. Reproducible polypeptide profiles obtained by sodium dodecyl sulphate (SDS)-capillary gel electrophoresi s displayed some peptides characteristic of G. rostochiensis and other s characteristic of G. pallida. Polypeptide profiles of each of the pa thotypes of G. rostochiensis Ro1, Ro2, Ro3, Ro4, Ro5, and G. pallida P al, Pa2/Pa3 were obtained. Profiles of Australian isolates from Victor ia were of the Ro1 type and this pathotype classification was also det ermined by standard differential cultivar tests. The CE technique outl ined is a reliable, reproducible technique which could aid with the di fferentiation of these pathotypes with minimum sample preparation. (C) Elsevier - ORSTOM.