RECOMBINANT EXPRESSION OF ALLIIN LYASE FROM GARLIC (ALLIUM-SATIVUM) IN BACTERIA AND YEASTS

Authors
WEIK R FRANCKY A STRIEDNER C RASPOR P BAYER K MATTANOVICH D
Citation
R. Weik et al., RECOMBINANT EXPRESSION OF ALLIIN LYASE FROM GARLIC (ALLIUM-SATIVUM) IN BACTERIA AND YEASTS, Planta medica, 64(4), 1998, pp. 387-388
Citations number
18
Categorie Soggetti
Pharmacology & Pharmacy","Plant Sciences","Chemistry Medicinal
Journal title
Planta medica → ACNP
ISSN journal
00320943
Volume
64
Issue
4
Year of publication
1998
Pages
387 - 388
Database
ISI
SICI code
0032-0943(1998)64:4<387:REOALF>2.0.ZU;2-I
Abstract
Recombinant garlic alliin lyase was produced in Escherichia coli, Sacc haromyces cerevisiae, and Pichia pastoris. A cDNA clone was obtained f rom garlic bulbs by PCR and introduced into suitable bacterial and yea st expression vectors. The recombinant alliin lyase forms inclusion bo dies in all three host organisms, which are deposited in the cytoplasm . After cell ly sis and harvesting by centrifugation, the inclusion bo dies were solubilized in Zwittergent 3-14 solution and refolded by ste pwise dilution. Specific alliin lyase activity could be recovered by t his procedure.