GLUCOCORTICOID EXPOSURE IN LATE-GESTATION PERMANENTLY PROGRAMS RAT HEPATIC PHOSPHOENOLPYRUVATE CARBOXYKINASE AND GLUCOCORTICOID RECEPTOR EXPRESSION AND CAUSES GLUCOSE-INTOLERANCE IN ADULT OFFSPRING
Mj. Nyirenda et al., GLUCOCORTICOID EXPOSURE IN LATE-GESTATION PERMANENTLY PROGRAMS RAT HEPATIC PHOSPHOENOLPYRUVATE CARBOXYKINASE AND GLUCOCORTICOID RECEPTOR EXPRESSION AND CAUSES GLUCOSE-INTOLERANCE IN ADULT OFFSPRING, The Journal of clinical investigation, 101(10), 1998, pp. 2174-2181
Low birth weight in humans is predictive of insulin resistance and dia
betes in adult life. The molecular mechanisms underlying this link are
unknown but fetal exposure to excess glucocorticoids has been implica
ted, The fetus is normally protected from the higher maternal levels o
f glucocorticoids by fete-placental 11 beta-hydroxysteroid dehydrogena
se type-2 (11 beta-HSD2) which inactivates glucocorticoids. We have sh
own previously that inhibiting 11 beta-HSD2 throughout pregnancy in ra
ts reduces birth weight and causes hyperglycemia in the adult offsprin
g. We now show that dexamethasone (a poor substrate for 11 beta-HSD2)
administered to pregnant rats selectively in the last week of pregnanc
y reduces birth weight by 10% (P < 0.05), and produces adult fasting h
yperglycemia (treated 5.3 +/- 0.3; control 4.3 +/- 0.2 mmol/liter, P =
0.04), reactive hyperglycemia (treated 8.7 +/- 0.4; control 7.5 +/- 0
.2 mmol/liter, P = 0.03), and hyperinsulinemia (treated 6.1 +/- 0.4; c
ontrol 3.8 +/- 0.5 ng/ml, P = 0.01) on oral glucose loading, In the ad
ult offspring of rats exposed to dexamethasone in late pregnancy, hepa
tic expression of glucocorticoid receptor (GR) mRNA and phosphoenolpyr
uvate carboxykinase (PEPCK) mRNA land activity) are increased by 25% (
P = 0.01) and 60% (P < 0.01), respectively, while other liver enzymes
(glucose-6-phosphatase, glucokinase, and 11 beta-hydroxysteroid dehydr
ogenase type-1) are unaltered. Tn contrast dexamethasone, when given i
n the first or second week of gestation, has no effect on offspring in
sulin/glucose responses or hepatic PEPCK and GR expression, The increa
sed hepatic GR expression may be crucial, since rats exposed to dexame
thasone in utero showed potentiated glucose responses to exogenous cor
ticosterone. These observations suggest that excessive glucocorticoid
exposure late in pregnancy predisposes the offspring to glucose intole
rance in adulthood, Programmed hepatic PEPCK overexpression, perhaps m
ediated by increased GR, may promote this process by increasing glucon
eogenesis.