INCREASED PLASMA P-SELECTIN INDUCED BY INTRAVENOUS ADMINISTRATION OF ENDOTOXIN IN RATS

We developed a competitive enzyme immunoassay for detection of rat P-s electin and examined temporal changes of plasma P-selectin levels in a n endotoxin-induced injury model in rats. Soluble P-selectin was detec ted in rat plasma after intravenous administration of lipopolysacchari de (LPS), and increased to a maximum level of five-fold over baseline after 24 hours. Plasma P-selectin was partially purified by gelfiltrat ion and was identified as a 122 kDa band by Western blotting. Using th e ELISA system we developed, monitoring of plasma P-selectin has becom e possible in the rat. C() 1998 Academic Press.