Ct. Christoffersen et al., INSULIN AND INSULIN-LIKE GROWTH-FACTOR-I RECEPTOR-MEDIATED DIFFERENTIATION OF 3T3-F442A CELLS INTO ADIPOCYTES - EFFECT OF PI 3-KINASE INHIBITION, Biochemical and biophysical research communications, 246(2), 1998, pp. 426-430
The ability of insulin and insulin-like growth factors (IGF-I and IGF-
II) to induce differentiation of 3T3-F442A cells into adipocytes was e
xamined at various hormone concentrations. Both insulin and the IGFs p
romoted differentiation at concentrations compatible with binding to t
heir cognate receptors, suggesting that both insulin and EGF-I recepto
rs are capable of promoting this differentiation. Adipocyte conversion
of 3T3-F442A cells was completely blocked in the presence of LY294002
, a specific inhibitor of PI 3-kinase, indicating that PI 3-kinase act
ivity plays a crucial role in the initial signalling events that trigg
er this differentiation process. (C) 1998 Academic Press.