INSULIN AND INSULIN-LIKE GROWTH-FACTOR-I RECEPTOR-MEDIATED DIFFERENTIATION OF 3T3-F442A CELLS INTO ADIPOCYTES - EFFECT OF PI 3-KINASE INHIBITION

Citation
Ct. Christoffersen et al., INSULIN AND INSULIN-LIKE GROWTH-FACTOR-I RECEPTOR-MEDIATED DIFFERENTIATION OF 3T3-F442A CELLS INTO ADIPOCYTES - EFFECT OF PI 3-KINASE INHIBITION, Biochemical and biophysical research communications, 246(2), 1998, pp. 426-430
Citations number
38
Categorie Soggetti
Biology,Biophysics
ISSN journal
0006291X
Volume
246
Issue
2
Year of publication
1998
Pages
426 - 430
Database
ISI
SICI code
0006-291X(1998)246:2<426:IAIGRD>2.0.ZU;2-0
Abstract
The ability of insulin and insulin-like growth factors (IGF-I and IGF- II) to induce differentiation of 3T3-F442A cells into adipocytes was e xamined at various hormone concentrations. Both insulin and the IGFs p romoted differentiation at concentrations compatible with binding to t heir cognate receptors, suggesting that both insulin and EGF-I recepto rs are capable of promoting this differentiation. Adipocyte conversion of 3T3-F442A cells was completely blocked in the presence of LY294002 , a specific inhibitor of PI 3-kinase, indicating that PI 3-kinase act ivity plays a crucial role in the initial signalling events that trigg er this differentiation process. (C) 1998 Academic Press.