A novel flavonoid diglycoside, 5,6,7,4'-tetrahydroxyflavonol 3-O-rutin
oside (1), and a previously known compound, kaempferol 3-O-neohesperid
oside (2), were isolated from an ethyl acetate extract of Daphniphyllu
m calycinum leaves that showed significant activity in a 1,1-diphenyl-
2-picrylhydrazyl (DPPH) free-radical assay. The structure of 1 was elu
cidated by a combination of spectroscopic methods, and compounds 1 and
2 were found to be moderately active as antioxidants in the DPPH assa
y.