INTRAHEPATIC LYMPHOCYTE ANALYSES AND ASSESSMENT OF THE EFFECTS OF LEVAMISOLE IN MURINE HEPATIC METASTASIS MODEL

We used hepatic metastasis models to determine the mechanism and effec t of levamisole. BALB/c mice and Colon 26 cells were used. Group I was injected with tumor cells through the portal vein. Group II was prime d with tumor cells before armor cells injecting. Group III was same as Group II, but treated with levamisole. Surface antigens of intrahepat ic lymphocytes and spleen cells were determined by FACScan with Anti-C D3, anti-CD4, anti-CD8, anti-CD45, anti-NK1.1 and anti-F4/80. Nodules on the liver were greatest in Group I and fewest in Group III. Concern ing intrahepatic lymphocytes, Group II, when compared with Group I, ha d ina eases of CD3(+), CD4(+) and CD8(+) cells, and decreases of CD45( +) and NK1.1(+) cells. Group III when compared with Group II, showed i ncreased CD8(+) cells and decreased of NK1.1(+) cells. Levamisole is c onsidered to be effective in the prevention of liver metastasis and is suggeste to enhanced CD8(+) cells.