DETERMINATION OF SERUM LEVELS OF 2,6 DIETHYLANILINE IN LABORATORY-ANIMAL TREATED WITH ALACHLOR

In ol der to estimate the environmental risk of the use of Alachlor, e xperiments on laboratory animals were conducted. Alachlor and 2,6 diet hylaniline content in blood serum was quantified. Three groups of male ACI/T rats and C3H/FEJ mice were treated with three different doses o f Alachlor. Six hours after the intraperitoneal injection the animals were bled and blood was collected by cardiac puncture. From serum obta ined after blood centrifugation, A and DEA were exacted using diethyl ether: 2,6 diethylaniline and Alachlor determinations were carried out by high performance liquid chromatography (HPLC). The HPLC revealed t hat the metabolic capacity of 2,6 diethylaniline production from Alach lor in rats is dose-dependent; moreover, the animals can be subdivided into at least two groups, according to their Alachlor metabolic capac ities. In mice the metabolic release of 2,6 diethylaniline was found t o be practically complete at every dose tested.