COMBINED EFFECTS OF TNP-470 AND TAXOL IN HUMAN NONSMALL CELL LUNG-CANCER CELL-LINES

The effects of TNP-470, one of the promising antiangiogenic agents and taxol when combined were evaluated in two human cultured cell lines d erived from non-small cell lung cancer (NSCLC). Cytotoxicity was deter mined using a tetrazolium salt (MTT) assay. The inhibitor effects of T NP-470 on cell proliferation were dose related and the 50% inhibitory concentration on these cell lines were 50.0 to 62.5 mu M. Evaluation o f drug interactions with isobologram and the combination index values showed that sequential exposure to taxol followed by TNP-470 produced synergistic effects in these cell lines tested Our findings suggest th at such an angiocytotoxic chemotherapy might be promising for the trea tment of NSCLC.