The effects of TNP-470, one of the promising antiangiogenic agents and
taxol when combined were evaluated in two human cultured cell lines d
erived from non-small cell lung cancer (NSCLC). Cytotoxicity was deter
mined using a tetrazolium salt (MTT) assay. The inhibitor effects of T
NP-470 on cell proliferation were dose related and the 50% inhibitory
concentration on these cell lines were 50.0 to 62.5 mu M. Evaluation o
f drug interactions with isobologram and the combination index values
showed that sequential exposure to taxol followed by TNP-470 produced
synergistic effects in these cell lines tested Our findings suggest th
at such an angiocytotoxic chemotherapy might be promising for the trea
tment of NSCLC.