M. Fujiwara et al., LUNG-CANCER CELL-LINE PRODUCING CYTOKERATIN-19 FRAGMENT AND SIALYL-LEWIS X-I ANTIGEN, Anticancer research, 18(2A), 1998, pp. 1043-1046
A new human lung large cell carcinoma cell line producing cytokeratin
19 fragment(CYFRA) and sialyl Lewis Xi antigen(SLX), designated TKB-13
, was established front a patent in our laboratory. The cell line grew
in a monolayer and had a polygonal epitheloid appearance in phase-con
trast microscopy. The doubling time of TKB-13 cells was approximately
32 hours in Dulbecco's minimal essential medium with 5% fetal bovine s
erum. TKB-13 cells could be transplanted to nude mice and the histolog
ical characteristics were the same as those of resected tissue from th
e primary site. Positive reactions for CYFRA and for SLX were seen wit
hin the cytoplasm of TKB-13 cells by immunocytochemical staining. Both
CYFRA and SLX were detected in the culture supernatant. Our results s
uggested that TKB-13 cell line is available for experimental studies o
n the biological behavior of non-small cell lung cancer.