FREE-RADICAL ERYTHROCYTE DAMAGE IN TUMORAL DISEASE ASSESSED BY HE-NE-LASER AND OPTICAL MICROSCOPE THROUGH HEINZ BODIES METHOD

Results obtained from blood sample readings by optical microscopy and He-Ne laser (lambda = 630,1 mW), have confirmed the reduction in Heinz Bodies (HB) formation lime and the Transmittance Reduction Degree (TR D), in malignancies. The results of spectrometric readings in colorect um polyposis, (TRD = 0,07) and fibrocystic mastopathy (TRD = 0,08) gav e results overlapping with controls (TRD = 0,08). In neoplasias, the e arly HE formation in erythrocytes observed by optical microscope corre sponded to TRD increase = 0,17 (P<0.01). TRD increase was statisticall y significant (P<0,01), as well as the reduction in the time of HB app earance (<0.01). The relationship between optical and laser readings w as exponential in tumors, while it was linear in controls, in polyposi s and mastopathies. The values of the correlation coefficients obtaine d by both methods were significant (P<0,01) for all the studied groups . Moreover, these research data further support the existence, even in the earlier stages of the disease, of the labile state of the red cel l membrane due to strong lipid peroxidation by FRs.