IDENTIFICATION OF CYTOCHROME P450S IN HUMAN GLIOMA CELL-LINE

Authors
VASQUEZ HG STROBEL H
Citation
Hg. Vasquez et H. Strobel, IDENTIFICATION OF CYTOCHROME P450S IN HUMAN GLIOMA CELL-LINE, International journal of oncology, 12(6), 1998, pp. 1291-1294
Citations number
29
Categorie Soggetti
Oncology
Journal title
International journal of oncology → ACNP
ISSN journal
10196439
Volume
12
Issue
6
Year of publication
1998
Pages
1291 - 1294
Database
ISI
SICI code
1019-6439(1998)12:6<1291:IOCPIH>2.0.ZU;2-J
Abstract
Five different isoforms of cytochrome P450 including 1A1, 1A2, 2E1, 2A and 2B6 have been identified in human glioma Hs 683 cell line using R T-PCR reaction. These isoforms belong to four distinct subfamilies. Th e effect of benzanthracene (Ba) as inducer was tested on the mRNA leve l of cytochrome P450 1A1. Northern blot analysis clearly showed an ind uction response from these cells to Ba in a proportion that is compara ble to the induction seen in rat glioma cells.