STRUCTURE OF THE O-ANTIGEN OF VIBRIO-CHOLERAE O155 THAT SHARES A PUTATIVE D-GALACTOSE 4,6-CYCLOPHOSPHATE-ASSOCIATED EPITOPE WITH V-CHOLERAEO139 BENGAL

The O-specific polysaccharide of Vibrio cholerae O155 was studied by s ugar and methylation analyses, dephosphorylation with 48% hydrofluoric acid, H-1- and C-13-NMR spectroscopy, including two-dimensional COSY, TOCSY, NOESY, and heteronuclear single-quantum coherence (HSQC) exper iments. The following structure of the pentasaccharide repeating unit of the polysaccharide was established: [GRAPHICS] An unusual component , D-galactose 4,6-cyclophosphate, has been reported previously as a co mponent of the capsular polysaccharide and O-antigen of Il cholerae O1 39 Bengal and appears to be responsible for the known serological cros s-reactivity between V. cholerae O139 and O155.