THE EFFECTS OF DURATION OF CO2 INSUFFLATION AND IRRIGATION ON PERITONEAL MICROCIRCULATION ASSESSED BY FREE-RADICAL SCAVENGERS AND TOTAL GLUTATHIONE LEVELS DURING OPERATIVE LAPAROSCOPY

Study Objective. To investigate the effects of peritoneal exposure to carbon dioxide (CO2) on peritoneal microcirculation and free radical s cavenger (FRS) metabolism, and its role in potential adhesion formatio n after operative laparoscopy. Design. Randomized, controlled study (C anadian Task Force classification I). Setting. University-affiliated h ospital. Patients. Twenty-eight women undergoing operative laparoscopy for adnexal masses. Intervention. For each patient, a 1 x I-cm sidewa ll peritoneal flap was excised at the end of laparoscopy and numbered randomly. Similar flaps obtained from 24 women immediately after enter ing the abdomen during laparotomy served as controls. Measurements and Main Results. Changes in glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH) levels were stu died in homogenized peritoneal tissues. The duration of CO2 exposure a nd amount of CO2 used were correlated with levels of free radical scav engers and compared with controls. Mean CO2 exposure, amount of CO2 us ed, and CO2 pressure (15 mm Hg) was similar between low irrigation and irrigated laparoscopy (118.3 +/- 25 and 39.2 +/- 8.81 min and 125 +/- 20 and 44.5 +/- 6.81 min, respectively). The change in FRS levels was significantly correlated with duration and amount of CO2 exposure ( r = -0.92). Levels of GSH-Px, SOD, CAT, and GSH were significantly lowe r in the CO2 exposure group than in controls (0.57 els oi GSH-Px, SOD, CAT; and GSH were significantly lower in the CO2 exposure group than in controls (0.57 mu mol, 1.8 ng, 48.5 mu mol, 1.5 nmol vs 0.8 mu mol, 2.6 +/- 0.4 ng, 79 mu mol, 3.6 nmol, respectively). Conclusion, Expos ure to CO2 has adverse effects on peritoneal microcirculation and cell -protective systems, which are proposed mechanisms in adhesion formati on. Avoiding long CO2 exposure and copiously irrigating the abdominal cavity throughout surgery may lessen these effects. The potential role oi the peritoneal FRS system on postoperative adhesion formation and its relation to estrogen status mandates further studies.