DESIGN AND OPTIMIZATION OF A NEAR-FIELD SCANNING OPTICAL MICROSCOPE FOR IMAGING BIOLOGICAL SAMPLES IN LIQUID

Authors
GHEBER LA HWANG J EDIDIN M
Citation
La. Gheber et al., DESIGN AND OPTIMIZATION OF A NEAR-FIELD SCANNING OPTICAL MICROSCOPE FOR IMAGING BIOLOGICAL SAMPLES IN LIQUID, Applied optics, 37(16), 1998, pp. 3574-3581
Citations number
30
Categorie Soggetti
Optics
Journal title
Applied optics → ACNP
ISSN journal
00036935
Volume
37
Issue
16
Year of publication
1998
Pages
3574 - 3581
Database
ISI
SICI code
0003-6935(1998)37:16<3574:DAOOAN>2.0.ZU;2-U
Abstract
We describe a near-field scanning optical microscope capable of imagin g biological samples in liquid. The microscope uses a straight optical fiber near-field probe and optical sheer-force feedback for tip sampl e distance regulation. Physical aspects of the design are discussed, a nd phenomena related to operation in liquid are revealed. Careful cali bration of the instrument in air and in liquid is shown, and for the f irst time to our knowledge, near-field fluorescence images of a biolog ical cell in liquid are presented. (C) 1998 Optical Society of America .