Y. Georgalis et al., HUNTINGTIN AGGREGATION MONITORED BY DYNAMIC LIGHT-SCATTERING, Proceedings of the National Academy of Sciences of the United Statesof America, 95(11), 1998, pp. 6118-6121
An initial stage of fibrillogenesis in solutions of glutathione S-tran
sferase-huntingtin (GST-HD) fusion proteins has been studied by using
dynamic light scattering. Two GST-HD systems with poly-L-glutamine (po
lyGln) extensions of different lengths (20 and 51 residues) have been
examined. For both systems, kinetics of z-average translation diffusio
n coefficients (D-app) and their angular dependence have been obtained
. Our data reveal that aggregation does occur in both GST-HD51 and GST
-HD20 solutions, but that it is much more pronounced in the former. Th
us, our approach provides a powerful tool for the quantitative assay o
f GST-HD fibrillogenesis in vitro.