HUNTINGTIN AGGREGATION MONITORED BY DYNAMIC LIGHT-SCATTERING

Authors
GEORGALIS Y STARIKOV EB HOLLENBACH B LURZ R SCHERZINGER E SAENGER W LEHRACH H WANKER EE
Citation
Y. Georgalis et al., HUNTINGTIN AGGREGATION MONITORED BY DYNAMIC LIGHT-SCATTERING, Proceedings of the National Academy of Sciences of the United Statesof America, 95(11), 1998, pp. 6118-6121
Citations number
28
Categorie Soggetti
Multidisciplinary Sciences
Journal title
Proceedings of the National Academy of Sciences of the United Statesof America → ACNP
ISSN journal
00278424
Volume
95
Issue
11
Year of publication
1998
Pages
6118 - 6121
Database
ISI
SICI code
0027-8424(1998)95:11<6118:HAMBDL>2.0.ZU;2-U
Abstract
An initial stage of fibrillogenesis in solutions of glutathione S-tran sferase-huntingtin (GST-HD) fusion proteins has been studied by using dynamic light scattering. Two GST-HD systems with poly-L-glutamine (po lyGln) extensions of different lengths (20 and 51 residues) have been examined. For both systems, kinetics of z-average translation diffusio n coefficients (D-app) and their angular dependence have been obtained . Our data reveal that aggregation does occur in both GST-HD51 and GST -HD20 solutions, but that it is much more pronounced in the former. Th us, our approach provides a powerful tool for the quantitative assay o f GST-HD fibrillogenesis in vitro.