Peroxidase (E.C.1.11.1.7) activity in twigs of American (Castanea dent
ata), Chinese (C. mollissima), and Japanese (C. crenata) chestnut was
determined monthly over an eight month period. The total amount of per
oxidase activity was highest in December and April for most trees. An
inhibitor of peroxidase activity was present in the extracts which cou
ld be removed by gel filtration. Extracts from healthy twigs had consi
stent major bands on IEF gels. However, a new band was detected in bar
k of American chestnut after wounding or infection with chestnut bligh
t fungus (Cryphonectria parasitica). (C) 1998 Elsevier Science Ltd. Al
l rights reserved.