STOPPED-FLOW ANALYSIS OF RU(BPY)(3)(3+) CHEMILUMINESCENT REACTIONS

The stopped-flow technique was employed to measure chemiluminescent em ission from the reaction of a mixture of oxalate and proline with a ch emiluminescence reagent, tris(2,2'-bipyridine)ruthenium(III), or Ru(bp y)(3)(3+). Ru(bpy)(3)(3+) is a versatile reagent and is often used in bioanalytical applications, including the detection of certain drugs a nd their metabolites, for example. Unfortunately, Ru(bpy)(3)(3+) has n ot yet been fully examined as a possible chemiluminescence reagent for simultaneous kinetic determinations. In this work, a differential rea ction rate method, based on simple least squares regressions of the ps eudo-first order decay data, was used to resolve two compounds, oxalat e and proline, reacting simultaneously with Ru(bpy)(3)(3+). Our result s indicate that stopped-flow analyses with Ru(bpy)(3)(3+) could provid e a viable method for simultaneous determinations of unresolvable anal ytes of environmental and pharmaceutical importance. (C) 1998 John Wil ey & Sons, Ltd.