KINETICS OF D-MALATE PRODUCTION BY PERMEABILIZED PSEUDOMONAS PSEUDOALCALIGENES

The Kinetics of D-malate production from maleate by permeabilized Pseu domonas pseudoalcaligenes was described by assuming competitive produc t inhibition in addition to biocatalyst inactivation. The kinetic para meters for conversion and inactivation were determined from a simultan eous fit of two independent data sets of substrate concentration vs, t ime. In order to select appropriate conditions, the effects of pH and temperature on the initial substrate conversion rate (excluding inacti vation) were studied first. The pH optimum ranged from 7-8. The temper ature optimum was approximately 45 degrees C. The conversion parameter s increased with pH and/or temperature. The first-order inactivation r ate constant depended on temperature only; it increased strongly betwe en 25-35 degrees C. (C) 1998 Elsevier Science Inc.