SEX DETERMINATION OF PARROTS AMAZONA-AMAZONIXA-AESTIVA AND AMAZONA-AMAZONICA USING THE POLYMERASE-CHAIN-REACTION

Authors
DEMATTOS KK DELLAMA SN SALLES ARC FRANCISCO MR MEDAGLIA A TOMASULO AM CARMO FMS PEREIRA JMC DEOLIVEIRA MA
Citation
Kk. Demattos et al., SEX DETERMINATION OF PARROTS AMAZONA-AMAZONIXA-AESTIVA AND AMAZONA-AMAZONICA USING THE POLYMERASE-CHAIN-REACTION, The Journal of experimental zoology, 281(3), 1998, pp. 217-219
Citations number
16
Categorie Soggetti
Zoology
Journal title
The Journal of experimental zoology → ACNP
ISSN journal
0022104X
Volume
281
Issue
3
Year of publication
1998
Pages
217 - 219
Database
ISI
SICI code
0022-104X(1998)281:3<217:SDOPAA>2.0.ZU;2-U
Abstract
A method was developed for sex determination in two parrot species (Am azona a. aestiva and Amazona amazonica). A protocol is described for d irect PCR amplification from parrot whole blood using primers designed for Xho I repetitive regions of chicken W-chromosome. A W-specific fr agment (approximate size of 124 bp) was amplified only in six DNA-fema le samples. No amplification was observed in 19 male samples. Complete correspondence was observed between our sex identification results an d those obtained with endoscopy, karyotype analyses, and behavioral ob servations. (C) 1998 Wiley-Liss, Inc.