CHARACTERIZATION OF 2 SP1 BINDING-SITES OF THE HUMAN SEX-DETERMINING SRY PROMOTER

Authors
DESCLOZEAUX M POULAT F BARBARA PD SOULLIER S JAY P BERTA P BOIZETBONHOURE B
Citation
M. Desclozeaux et al., CHARACTERIZATION OF 2 SP1 BINDING-SITES OF THE HUMAN SEX-DETERMINING SRY PROMOTER, Biochimica et biophysica acta, N. Gene structure and expression, 1397(3), 1998, pp. 247-252
Citations number
31
Categorie Soggetti
Biology,Biophysics
Journal title
Biochimica et biophysica acta, N. Gene structure and expression → ACNP
ISSN journal
01674781
Volume
1397
Issue
3
Year of publication
1998
Pages
247 - 252
Database
ISI
SICI code
0167-4781(1998)1397:3<247:CO2SBO>2.0.ZU;2-K
Abstract
To investigate the molecular basis of the human SRY gene regulation, w e have examined the significance of two potential binding sites for th e transcription factor Sp1 (Sp1A: -124 to -131 and Sp1B: -147 to -154) by DNase I footprinting and gel mobility shift assays. Cotransfection experiments in Drosophila SL2 cells implicated Spl protein in the tra nscriptional activation of the SRY promoter. (C) 1998 Elsevier Science B.V.