Structures of two short-lived intermediates during the photocycle of t
he photoactive yellow protein (PYP) have been determined using X-ray c
rystallography. These structures demonstrate the power of cryo-trappin
g and the Laue method in trapping transient intermediates.Structures o
f two short-lived intermediates during the photocycle of the photoacti
ve yellow protein (PYP) have been determined using X-ray crystallograp
hy. These structures demonstrate the power of cryo-trapping and the La
ue method in trapping transient intermediates.