EFFECT OF SURFACE-ACTIVE COMPOUNDS ON GRO WTH AND ADHESION OF ANCHORAGE-DEPENDENT ANIMAL-CELLS AT LIQUID LIQUID INTERFACE/

As a novel cell cultivation method, cell cultivation using a liquid/li quid interface system (culture medium/fluorocarbon) has been investiga ted. Cell adhesion at the interface appears to be dependent on the con taminants contained in the fluorocarbon. Tn this study, the effect of surfactant on the growth and adhesion of the cells was studied. Althou gh the cells could not anchor at the interface between nonionic surfac tant-added medium and fluorocarbon, cell growth was observed when ioni c surfactant, instead of nonionic surfactant, was used. When ionic sur factant was added to fluorocarbon, cell growth was better than that at the interface between ionic surfactant-added medium and fluorocarbon. In particular, the cell growth at the interface using fluorocarbon ad ded pentafluorobenzyl chloride or perfluorooctanoyl chloride was simil ar to that at the polystyrene surface, which indicates that it is impo rtant to add the surfactant to fluorocarbon.