CHIRAL SEPARATION OF DL-TRYPTOPHAN USING BOVINE-SERUM-ALBUMIN-MULTILAYERED POROUS HOLLOW-FIBER MEMBRANE

A diethylamino (DEA) -group-containing polymer chain was grafted onto a porous polyethylene membrane in the form of a hollow fiber by radiat ion-induced graft polymerization of an epoxy-group containing vinyl mo nomer (GMA), followed by a reaction of the epoxy group with diethylami ne. Bovine serum albumin (BSA) was multilayered up to six layers by th e permeation of a BSA solution through the pores of the DEA -group-con taining porous membrane. A chromatogram of DL-tryptophan was obtainabl e by the injection of a racemic Trp solution into a BSA-multilayered m embrane acting as a stationary phase. The selectivity coefficient, def ined as the retention time ratio of L-Trp to D-Trp, increased with the degree of BSA multilayer binding, and remained constant irrespective of the flow rate of the mobile phase because of a negligible diffusion al mass-transfer resistance.