CHROMOSOMAL LOCALIZATION OF THE 5-HT1F RECEPTOR GENE - NO EVIDENCE FOR INVOLVEMENT IN RESPONSE TO SUMATRIPTAN IN MIGRAINE PATIENTS

The 5-HT1F receptor, which is present in both human vascular and neuro nal tissue, may mediate the therapeutic effect and/or side-effects of sumatriptan, We investigated the chromosomal localization of the 5-HT1 F receptor gene and the relation between eventually existing polymorph isms and the clinical response to sumatriptan in migraine patients. Th e 5-HT1F receptor gene was localized using a monochromosomal mapping p anel, followed by a radiation-reduced hybrid mapping and fluorescent i n situ hybridization. The results of these techniques show that the 5- HT1F receptor gene is localized at 3p12. We investigated the presence of polymorphisms by single strand conformation polymorphism analysis i n 14 migraine patients who consistently responded well to sumatriptan, 12 patients who consistently experienced recurrence of the headache a fter initial relief, 12 patients with no response to sumatriptan, and in 13 patients who consistently experienced chest symptoms after use o f sumatriptan. No polymorphisms were detected in any of the patients. We therefore conclude that genetic diversity of the 5-HT1F receptor ge ne is most probably not responsible for the variable clinical response to sumatriptan. (C) 1998 Wiley-Liss, Inc.