OXIDATION OF PAH AND PAH-DERIVATIVES BY FUNGAL AND PLANT OXIDOREDUCTASES

Citation
T. Gunther et al., OXIDATION OF PAH AND PAH-DERIVATIVES BY FUNGAL AND PLANT OXIDOREDUCTASES, Journal of basic microbiology, 38(2), 1998, pp. 113-122
Citations number
41
Categorie Soggetti
Microbiology
ISSN journal
0233111X
Volume
38
Issue
2
Year of publication
1998
Pages
113 - 122
Database
ISI
SICI code
0233-111X(1998)38:2<113:OOPAPB>2.0.ZU;2-7
Abstract
The in vitro-oxidation of the three-and four-ring polycyclic aromatic hydrocarbons (PAH) anthracene, phenanthrene, pyrene and fluoranthene b y preparations of extracellular lignin peroxidase (LiP), manganese per oxidase (MnP) and laccase (Lacc) of the white rot fungus Nematoloma fr owardii and by mushroom tyrosinase (Tyr) and horseradish peroxidase (H RP) was investigated. LiP transformed 58.6% of anthracene and 34.2% of pyrene, whereas 31.5% of anthracene and 11.2% pyrene were oxidized by MnP. In the presence of the mediating substances veratryl alcohol (fo r LiP), GSH (for MnP), and ABTS (for Lace, Tyr, HRP), the conversion o f PAH was enhanced in most cases. Inclusion of PAM-derivatives, known as intermediates or potential dead-end-products of microbial PAH metab olism, in the in vitro-oxidation studies, demonstrated that the hydrox ylated PAH metabolites served as substrates for all oxidoreductases te sted, whereas PAH-quinones and ore-metabolites were not transformed.