ESTIMATION OF NITRIFYING BACTERIAL ACTIVITIES BY MEASURING OXYGEN-UPTAKE IN THE PRESENCE OF THE METABOLIC-INHIBITORS ALLYLTHIOUREA AND AZIDE

Authors
GINESTET P AUDIC JM URBAIN V BLOCK JC
Citation
P. Ginestet et al., ESTIMATION OF NITRIFYING BACTERIAL ACTIVITIES BY MEASURING OXYGEN-UPTAKE IN THE PRESENCE OF THE METABOLIC-INHIBITORS ALLYLTHIOUREA AND AZIDE, Applied and environmental microbiology, 64(6), 1998, pp. 2266-2268
Citations number
19
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
64
Issue
6
Year of publication
1998
Pages
2266 - 2268
Database
ISI
SICI code
0099-2240(1998)64:6<2266:EONBAB>2.0.ZU;2-B
Abstract
The effects of two metabolic inhibitors on an enriched nitrifying biom ass during incubation for short periods of time were investigated by d etermining respirometric measurements. Allylthiourea (86 mu M) and azi de (24 mu M) were shown to he strong, selective inhibitors of ammonia and nitrite oxidation, respectively. Consequently, a differential resp irometry method for estimating nitrifying and heterotrophic bacterial activities within a mixed biomass is proposed.