METHOD DETECTION LIMITS OF PCR AND IMMUNOFLUORESCENCE ASSAY FOR CRYPTOSPORIDIUM-PARVUM IN SOIL

Authors
WALKER MJ MONTEMAGNO C BRYANT JC GHIORSE WC
Citation
Mj. Walker et al., METHOD DETECTION LIMITS OF PCR AND IMMUNOFLUORESCENCE ASSAY FOR CRYPTOSPORIDIUM-PARVUM IN SOIL, Applied and environmental microbiology, 64(6), 1998, pp. 2281-2283
Citations number
12
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
Journal title
Applied and environmental microbiology → ACNP
ISSN journal
00992240
Volume
64
Issue
6
Year of publication
1998
Pages
2281 - 2283
Database
ISI
SICI code
0099-2240(1998)64:6<2281:MDLOPA>2.0.ZU;2-N
Abstract
We determined and compared the method detection limits (MDLalpha) of a PCR and an immunofluorescence assay (IFA) for detection of Cryptospor idium parvum oocysts in soils. Based on the MDLalpha and the quantitat ive nature and stability of the IFA, PCR analysis is not a useful scre ening step for soil studies of oocyst transport.