E. Feyzi et al., AGE-DEPENDENT MODULATION OF HEPARAN-SULFATE STRUCTURE AND FUNCTION, The Journal of biological chemistry, 273(22), 1998, pp. 13395-13398
Heparan sulfate interacts with growth factors, matrix components, effe
cters and modulators of enzymatic catalysis as well as with microbial
proteins via sulfated oligosaccharide domains. Although a number of su
ch domains have been characterized, little is known about the regulati
on of their formation in vivo. Here we show that the structure of huma
n aorta heparan sulfate is gradually modulated during aging in a manne
r that gives rise to markedly enhanced binding to isoforms of platelet
-derived growth factor A and B chains containing polybasic cell retent
ion sequences. By contrast, the binding to fibroblast growth factor 2
is affected to a much lesser extent. The enhanced binding of aorta hep
aran sulfate to platelet-derived growth factor is suggested to be due
to an age-dependent increase of GlcN 6-O-sulfation, resulting in incre
ased abundance of the trisulfated L-iduronic acid (2-OSO3)-GlcNSO(3)(6
-OSO3) disaccharide unit. Such units have been shown to hallmark the p
latelet-derived growth factor A chain-binding site in heparan sulfate.