Ch. Guo et al., DYNAMICS OF PROTEIN-KINASE CK2 ASSOCIATION WITH NUCLEOSOMES IN RELATION TO TRANSCRIPTIONAL ACTIVITY, The Journal of biological chemistry, 273(22), 1998, pp. 13675-13680
Protein kinase CK2 has been implicated in control of cell growth and p
roliferation. Since growth stimuli evoke its preferential association
with chromatin and nuclear matrix, we examined the dynamics of CK2 in
nucleosomes fractionated on the basis of their transcriptional activit
y in the rat prostate. In this model, androgens induce expression of a
ndrogen-dependent genes but inhibit the androgen-repressed genes, wher
eas absence of androgens has the reverse effect. The level of CK2 was
higher in the active than in inactive nucleosomes from normal prostate
. Differential alterations in the levels of CK2 activity in the transc
riptionally active versus inactive nucleosomes were evoked by androgen
deprivation or administration. Comparison of the distribution of CK2
in active and inactive nucleosomes under varying androgenic conditions
showed that the relative CK2 activity intrinsic to the transcriptiona
lly active nucleosomes remained fairly stable, concordant with gene ac
tivity specific to the androgenic status. However, CK2 associated with
inactive nucleosomes declined to a minimal level on androgen deprivat
ion but increased rapidly on androgen administration (reflecting expre
ssion of multiple androgen-dependent genes). We suggest a role for CK2
in promoting the conformational transition of inactive nucleosomes to
the active form and in the function of transcriptionally active nucle
osomes.