CHARACTERISTICS OF BENZODIAZEPINE RECEPTO RS IN THE CEREBELLUM OF RATS 6 MONTHS AFTER CORAZOL KINDLING AND REPEATED SEIZURES

It was shown that the increased brain seizure readiness persisted with in 6 months after termination of corazol kindling. Seizures of the sam e severity as during kindling (corazol injection in a dose of 20 mg/kg ) were reproduced by corazol injection in a dose of 30 mg/kg. In contr ast to the control rats, in this situation an autoenhancement of seizu res was observed in the kindled animals. Acute corazol seizures induce d a decrease in Bmax and Kd of H-3-diazepam dinding with benzodiazepin e receptors (BDR) in the cerebellum of the 10-months-old control rats white the young animals demonstrated only a decrease in Bmax of bindin g. In 6 months after kindling termination the BDR activity (Bmax) was reduced by one half. However, we think that the increase in Bmax is no t responsible for persistence of the increased seizure readiness. It s eems possible that down regulation of receptor activity develops indep endently of kindling but in response to long-lasting corazol applicati on. Probably, Bmax spontaneously decreases after the termination of th e long-term corazol application. The single dose of corazol (30 mg/kg) restores the changes in BDR density to the level when seizure readine ss has been just fixed (6 months after kindling termination), independ ently of the primary receptor density.