POLLEN-SPECIFIC GLUTAMATE-DEHYDROGENASE (GDH-II) IS OBSERVED AFTER MICROSPORE MITOSIS IN CAPSICUM-ANNUUM-L

A pollen-specific glutamate dehydrogenase has been characterized durin g microsporogenesis in Capsicum annuum L. Polyacrylamide gel electroph oresis of anther produces two isozymes, GDH I and GDH II. The presence of GDH I is observed in the sporophytic tissues of the plant includin g anthers and ovaries; hence, GDH I is considered as sporophyte-specif ic. Expression of GDH II is found only in pollen grains; therefore, GD H II is not only gametophyte-specific but also pollen-specific. Pollen -specific GDH II is observed after microspore mitosis and reaches maxi mum at the time of anthesis.