ANALYTICAL STRATEGIES FOR AMPEROMETRIC BIOSENSORS BASED ON CHEMICALLY-MODIFIED ELECTRODES

Various strategies based on the use of chemically modified electrodes for the development of amperometric biosensors are described, particul ar emphasis is placed on materials capable of catalyzing the oxidation of NADH and coupling these with enzymatic activities for biosensor co nstruction. In terms of electrocatalysts, the discussion will centre o n electrodeposited films of 3,4-dihydroxy benzaldehyde (3,4-DHB) and r elated analogs as well as on electrodeposited films of transition meta l complexes of 1,10-phenanthroline-5,6-dione (phen-dione). Electrodepo sited films of these materials have been coupled to the enzymatic acti vity of aldehyde dehydrogenase and alcohol dehydrogenase for the devel opment of biosensors for aldehydes and ethanol, respectively. (C) 1998 Elsevier Science S.A. All rights reserved.