ASSOCIATION OF PHENOTYPIC CHANGES IN B-CELL LYMPHOCYTES AND PLASMA VIRAL LOAD IN HUMAN IMMUNODEFICIENCY VIRUS-INFECTED PATIENTS

Many B cell abnormalities have been reported in human immunodeficiency virus (HIV)-infected patients, including changes in the expression of mu, gamma, and CD22 molecules on the cell surface. Phenotypic changes in these markers on B cells isolated from HIV-seropositive patients w ith high or low levels of plasma viremia were measured. The phenotypic changes in B cells isolated from such patients were compared with the markers on B cells isolated from HIV-seronegative individuals using t hree-color flow cytometry. HIV patients showed a reduction in the prop ortion of mature B cells isolated from peripheral blood mononuclear ce lls compared with B cells isolated from HIV-seronegative individuals. An increase in the proportion of B cells expressing both mu and gamma molecules on the cell surface was also seen in association with high-H IV plasma viremia. A low plasma viral load was accompanied by a reduct ion in the proportion of B cells expressing both mu and gamma molecule s to a level comparable to those seen in HIV-seronegative individuals. HIV-seropositive individuals demonstrated an increase in the proporti on of committed B cells, as indicated by an increase in the proportion of B cells expressing gamma molecules. This observation may explain t he poor humoral response of HIV seropositive patients to neo-antigens. Our results demonstrate that phenotypic changes indicative of in vivo B cell activation and an increase in immature cells are associated wi th HIV infection, particularly with a high plasma viral load. Phenotyp ic changes in B cell markers may correlate with functional deficits of B cells.