AN ORTHOGONAL SOLID-PHASE SUPPORT FOR THE SYNTHESIS OF OLIGONUCLEOTIDES CONTAINING 3'-PHOSPHATES AND ITS APPLICATION IN THE PREPARATION OF PHOTOLABILE HYBRIDIZATION PROBES

Authors
MCMINN DL HIRSCH R GREENBERG MM
Citation
Dl. Mcminn et al., AN ORTHOGONAL SOLID-PHASE SUPPORT FOR THE SYNTHESIS OF OLIGONUCLEOTIDES CONTAINING 3'-PHOSPHATES AND ITS APPLICATION IN THE PREPARATION OF PHOTOLABILE HYBRIDIZATION PROBES, Tetrahedron letters, 39(24), 1998, pp. 4155-4158
Citations number
18
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Tetrahedron letters → ACNP
ISSN journal
00404039
Volume
39
Issue
24
Year of publication
1998
Pages
4155 - 4158
Database
ISI
SICI code
0040-4039(1998)39:24<4155:AOSSFT>2.0.ZU;2-E
Abstract
Photolabile oligonucleotide synthesis supports (3) can be used to prep are biopolymers containing 3'-phosphates in good yield. When 3 is prep ared using Tentagel(R), oligonucleotides can be deprotected using stan dard concentrated aqueous ammonia conditions without cleaving the biop olymer from the support. The support bound oligonucleotide can capture its complement, and discriminate between a one base pair mismatch. Fo llowing photocleavage, the respective duplex is isolable by gel electr ophoresis. (C) 1998 Elsevier Science Ltd. All rights reserved.